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NEK2, Active recombinant protein

NEK, Serine/threonine-protein kinase Nek6

Product info
Primary Accession P51955
Concentration 0.1
Calculated MW 76.0 kDa
Additional Info
Gene ID 4751
Gene Symbol NEK2
Other Names NEK, Serine/threonine-protein kinase Nek6, Serine/threonine-protein kinase Nek6, Never in mitosis A-related kinase 6, NimA-related protein kinase 6, Protein kinase SID6-1512
Source Baculovirus (Sf9 insect cells)
Assay&Purity SDS-PAGE; ≥90%
Assay2&Purity2 HPLC;
Recombinant Yes
Format Liquid
Storage -80°C; Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 30% glycerol).
Citations (0)

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Nek 2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans that is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulated progressively throughout S, reaching maximal levels in late G2 (1). These observations demonstrate that Nek2 resembles Aspergillus NIMA, not only in its catalytic domain, but also in its cell cycle-dependent expression. Recombinant Nek2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation. Both human Nek2 and fungal NIMA phosphorylate a similar, albeit not identical, set of proteins and synthetic peptides, and β-casein is a suitable substrate for assaying Nek2 in vitro (2). Nek2 is shown to be expressed most abundantly in the testis of the adult tissues examined (3). Its expression in the testis is restricted to the germ cells, with highest levels detected in spermatocytes at pachytene and diplotene stages. Immunohistochemical analysis revealed that Nek2 localized to nuclei, exhibiting a non-uniform distribution within the nucleus.


Schultz S.J.,et al.Cell Growth Differ. 5:625-635(1994).
Hames R.S.,et al.Biochem. J. 361:77-85(2002).
Lu K.P.,et al.Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
Cat# PBV11295r
(40 western blots)
Availability: 5-7days
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