|Concentration||0.1 µg/ µl|
|Calculated MW||65.0 kDa|
|Other Names||PKA, cAMP-dependent protein kinase catalytic subunit gamma|
|Source||Baculovirus (Sf9 insect cells)|
|Storage||-80°C; Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.|
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Provided below are standard protocols that you may find useful for product applications.
PKA C-γ (PKAcg) is a third isoform of the catalytic subunit of cAMP-dependent protein kinase. It was isolated from a human testis cDNA library and was clearly derived from a gene distinct from C-α and C-β and showed tissue-specific expression. Whereas at the amino acid level C-α and C-β showed 93% homology, C-γ showed only about 80% homology to both C-α and C-β (1). The PRKACG gene is intronless, contains remnants of a poly (A) tail, is flanked by direct repeats, and is co-linear with the PRKACA gene(2).
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