PKCeta, Active recombinant protein
PKC, PKC, Protein kinase C eta
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P24723 |
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Concentration | 0.1 |
Calculated MW | 103.0 kDa |
Gene ID | 5583 |
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Gene Symbol | PRKCH |
Other Names | PKC, PKC, Protein kinase C eta |
Source | Baculovirus (Sf9 insect cells) |
Assay&Purity | SDS-PAGE; ≥90% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Format | Liquid |
Storage | -80°C; Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol). |
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Provided below are standard protocols that you may find useful for product applications.
Background
PKC eta is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases that can phosphorylate a wide variety of protein targets known to be involved in diverse cellular signaling pathways. PKC η is predominantly expressed in squamous cell epithelia and induces terminal differentiation of keratinocytes. PKC η that is endogenously expressed or overexpressed is found to associate with the cyclin E/cdk2/p21 complex in keratinocytes of mice and humans (1).
References
Bacher N.,et al.Mol. Cell. Biol. 11:126-133(1991).
Bacher N.,et al.Mol. Cell. Biol. 12:1404-1404(1992).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Heilig R.,et al.Nature 421:601-607(2003).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
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