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CHK1, Active recombinant protein

CHK, Serine/threonine-protein kinase Chk1

Product info
Primary Accession O14757
Concentration 0.1
Calculated MW 82.0 kDa
Additional Info
Gene ID 1111
Gene Symbol CHK1
Other Names CHK, Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1
Source Baculovirus (Sf9 insect cells)
Assay&Purity SDS-PAGE; ≥90%
Assay2&Purity2 HPLC;
Recombinant Yes
Format Liquid
Storage -80°C; Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 30% glycerol.).
Citations (0)

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CHK1 is a 56 kd serine/threonine protein kinase that was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle (1). CHK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia (2). In vitro, CHK1 binds to and phosphorylates the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases (3). CHK1 phosphorylates Cdc25C on serine-216 which creates a binding site for 14-3-3 protein and inhibits function of the phosphatase. Thus, in response to DNA damage, CHK1 phosphorylates and inhibits Cdc25C, thereby preventing the activation of the Cdc2-cyclin B complex and mitotic entry.


Sanchez Y.,et al.Science 277:1497-1501(1997).
Flaggs G.,et al.Curr. Biol. 7:977-986(1997).
Semba S.,et al.Int. J. Oncol. 16:731-737(2000).
Pabla N.,et al.Proc. Natl. Acad. Sci. U.S.A. 109:197-202(2012).
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 3,950.00
$ 295.00
Cat# PBV11310r
(40 western blots)
Availability: 5-7days
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