MAPKAPK2, Active recombinant protein
MAPKAPK, MAP kinase-activated protein kinase 2
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P49137 |
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Concentration | 0.1 |
Calculated MW | 68.0 kDa |
Gene ID | 9261 |
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Gene Symbol | MAPKAPK2 |
Other Names | MAPKAPK, MAP kinase-activated protein kinase 2 |
Source | Baculovirus (Sf9 insect cells) |
Assay&Purity | SDS-PAGE; ≥90% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Format | Liquid |
Storage | -80°C; Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol). |
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Provided below are standard protocols that you may find useful for product applications.
Background
MAPKAP kinase2 is a Ser/Thr protein kinase, which is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo.
References
Zu Y.-L.,et al.Biochem. Biophys. Res. Commun. 200:1118-1124(1994).
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Stokoe D.,et al.Biochem. J. 296:843-849(1993).
Jakob U.,et al.J. Biol. Chem. 268:1517-1520(1993).
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