|Calculated MW||53.4 kDa|
|Other Names||Glucokinase, Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D|
|Gene Source||Human liver|
|Storage||-80°C; 50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.|
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Provided below are standard protocols that you may find useful for product applications.
Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human liver Glucokinase (Accession# NP_277042)) has a C-terminal FLAG tag and has 474 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.
Tanizawa Y.,et al.Mol. Endocrinol. 6:1070-1081(1992).
Tanizawa Y.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:7294-7297(1991).
Nishi S.,et al.Diabetologia 35:743-747(1992).
Koranyi L.I.,et al.Diabetes 41:807-811(1992).
Stoffel M.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:7698-7702(1992).
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