Glucokinase, human pancreatic, recombinant protein
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P35557 |
---|---|
Concentration | 39.3 |
Calculated MW | 53.1 kDa |
Gene ID | 2645 |
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Gene Symbol | GCK |
Other Names | Glucokinase, Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D |
Gene Source | Human pancreas |
Source | E.coli |
Assay&Purity | SDS-PAGE; ≥80% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Format | Liquid |
Storage | -80°C; 50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human pancreatic Glucokinase (Accession# NP_000153)) has a C-terminal FLAG tag and has 470 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.
References
Tanizawa Y.,et al.Mol. Endocrinol. 6:1070-1081(1992).
Tanizawa Y.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:7294-7297(1991).
Nishi S.,et al.Diabetologia 35:743-747(1992).
Koranyi L.I.,et al.Diabetes 41:807-811(1992).
Stoffel M.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:7698-7702(1992).
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