|Calculated MW||39.0 kDa (358 aa, 1-338 aa + His Tag)|
|Other Names||Fructose-1, 6-bisphosphatase 1, FBPase1, FBP, FBP1|
|Results||Specific activity is > 0.30 unit/ml|
|Sequence||MGSSHHHHHH SSGLVPRGSH MADQAPFDTD VNTLTRFVME EGRKARGTGE LTQLLNSLCT AVKAISSAVR KAGIAHLYGI AGSTNVTGDQ VKKLDVLSND LVMNMLKSSF ATCVLVSEED KHAIIVEPEK RGKYVVCFDP LDGSSNIDCL VSVGTIFGIY RKKSTDEPSE KDALQPGRNL VAAGYALYGS ATMLVLAMDC GVNCFMLDPA IGEFILVDKD VKIKKKGKIY SLNEGYARDF DPAVTEYIQR KKFPPDNSAP YGARYVGSMV ADVHRTLVYG GIFLYPANKK SPNGKLRLLY ECNPMAYVME KAGGMATTGK EAVLDVIPTD IHQRAPVILG SPDDVLEFLK VYEKHSAQ|
|Storage||-20°C; 1 mg/ml solution 20 mM Tris-HCl buffer, pH 8.0, 10% glycerol and 1 mM DTT.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Fructose-1, 6-bisphosphatase 1(FBP1) is a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1, 6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1, 6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. Recombinant FBP1 protein, fused to His-tag at N-terminus, was expressed in E.Coli and purified by using conventional chromatography techniques.
Solomon D.H.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:6904-6908(1988).
El-Maghrabi M.R.,et al.J. Biol. Chem. 268:9466-9472(1993).
Kikawa Y.,et al.Biochem. Biophys. Res. Commun. 199:687-693(1994).
El-Maghrabi M.R.,et al.Genomics 27:520-525(1995).
Skalecki K.,et al.Arch. Biochem. Biophys. 365:1-9(1999).
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