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FBP1, human recombinant protein

Fructose-1,6-bisphosphatase 1, FBPase1, FBP, FBP1

     
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Product info
Primary Accession P09467
Concentration 1
Calculated MW 39.0 kDa (358 aa, 1-338 aa + His Tag)
Additional Info
Gene ID 2203
Gene Symbol FBP1
Other Names Fructose-1, 6-bisphosphatase 1, FBPase1, FBP, FBP1
Gene Source Human
Source E.coli
Assay&Purity SDS-PAGE; ≥90%
Assay2&Purity2 N/A;
Recombinant Yes
Results Specific activity is > 0.30 unit/ml
Sequence MGSSHHHHHH SSGLVPRGSH MADQAPFDTD VNTLTRFVME EGRKARGTGE LTQLLNSLCT AVKAISSAVR KAGIAHLYGI AGSTNVTGDQ VKKLDVLSND LVMNMLKSSF ATCVLVSEED KHAIIVEPEK RGKYVVCFDP LDGSSNIDCL VSVGTIFGIY RKKSTDEPSE KDALQPGRNL VAAGYALYGS ATMLVLAMDC GVNCFMLDPA IGEFILVDKD VKIKKKGKIY SLNEGYARDF DPAVTEYIQR KKFPPDNSAP YGARYVGSMV ADVHRTLVYG GIFLYPANKK SPNGKLRLLY ECNPMAYVME KAGGMATTGK EAVLDVIPTD IHQRAPVILG SPDDVLEFLK VYEKHSAQ
Target/Specificity FBP1
Format Liquid
Storage -20°C; 1 mg/ml solution 20 mM Tris-HCl buffer, pH 8.0, 10% glycerol and 1 mM DTT.
Citations (0)

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Background

Fructose-1, 6-bisphosphatase 1(FBP1) is a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1, 6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1, 6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. Recombinant FBP1 protein, fused to His-tag at N-terminus, was expressed in E.Coli and purified by using conventional chromatography techniques.

References

Solomon D.H.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:6904-6908(1988).
El-Maghrabi M.R.,et al.J. Biol. Chem. 268:9466-9472(1993).
Kikawa Y.,et al.Biochem. Biophys. Res. Commun. 199:687-693(1994).
El-Maghrabi M.R.,et al.Genomics 27:520-525(1995).
Skalecki K.,et al.Arch. Biochem. Biophys. 365:1-9(1999).

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$ 275.00
Cat# PBV11362r-100
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