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GPT/ALT1, human recombinant protein

Alanine aminotransferase 1, AAT1, ALT1, GPT1

     
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Product info
Primary Accession P24298
Concentration 0.5
Calculated MW 56.8 kDa (516 aa, 1-496 aa + His Tag)
Additional Info
Gene ID 2875
Gene Symbol GPT
Other Names Alanine aminotransferase 1, AAT1, ALT1, GPT1
Gene Source Human
Source E.coli
Assay&Purity SDS-PAGE; ≥90%
Assay2&Purity2 N/A;
Recombinant Yes
Results Specific activity is > 20 unit/mg
Sequence MGSSHHHHHH SSGLVPRGSH MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYS
Target/Specificity GPT/ALT1
Format Liquid
Storage -20°C; 0.5 mg/ml solution 20 mM Tris-HCl buffer, pH 8.0, 20% glycerol and 1 mM DTT.
Citations (0)

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Background

GPT, also known as alanine aminotransferases (ALT1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. This protein plays a key role in the intermediary metabolism of glucose and amino acids. It is widely used as an index of liver integrity or hepatocellular damage in clinical settings. Recombinant human GPT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

References

Sohocki M.M.,et al.Genomics 40:247-252(1997).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Ishiguro M.,et al.Biochemistry 30:10451-10457(1991).
Funatsu M.,et al.Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases.

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$ 375.00
Cat# PBV11364r-50
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