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GOT1, human recombinant protein

Glutamic-oxaloacetic transaminase 1, Aspartate aminotransferase 1, Transaminase A, GIG18

     
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Product info
Primary Accession P17174
Concentration 0.5
Calculated MW 48.4 kDa (433 aa, 1-413 aa + His Tag)
Additional Info
Gene ID 2805
Gene Symbol GOT1
Other Names Glutamic-oxaloacetic transaminase 1, Aspartate aminotransferase 1, Transaminase A, GIG18
Gene Source Human
Source E.coli
Assay&Purity SDS-PAGE; ≥95%
Assay2&Purity2 N/A;
Recombinant Yes
Results Specific activity is > 13 unit/mg
Sequence MGSSHHHHHH SSGLVPRGSH MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID PTPEQWKQIA SVMKHRFLFP FFDSAYQGFA SGNLERDAWA IRYFVSEGFE FFCAQSFSKN FGLYNERVGN LTVVGKEPES ILQVLSQMEK IVRITWSNPP AQGARIVAST LSNPELFEEW TGNVKTMADR ILTMRSELRA RLEALKTPGT WNHITDQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINV SGLTTKNLDY VATSIHEAVT KIQ
Target/Specificity GOT1
Format Liquid
Storage -20°C; 0.5 mg/ml solution 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 100 mM NaCl and 1 mM DTT.
Citations (0)

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Background

Glutamic-oxaloacetic transaminase (GOT) is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

References

Bousquet-Lemercier B.,et al.Biochemistry 29:5293-5299(1990).
Wang C.Y.,et al.Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
Yu W.,et al.Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 429:375-381(2004).

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$ 275.00
Cat# PBV11367r
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