|Calculated MW||48.4 kDa (433 aa, 1-413 aa + His Tag)|
|Other Names||Aspartate aminotransferase 2, mitochondrial, KAT4, KATIV, mitAAT|
|Results||Specific activity is > 44 unit/mg|
|Sequence||MGSSHHHHHH SSGLVPRGSH MGSSSWWTHV EMGPPDPILG VTEAFKRDTN SKKMNLGVGA YRDDNGKPYV LPSVRKAEAQ IAAKNLDKEY LPIGGLAEFC KASAELALGE NSEVLKSGRF VTVQTISGTG ALRIGASFLQ RFFKFSRDVF LPKPTWGNHT PIFRDAGMQL QGYRYYDPKT CGFDFTGAVE DISKIPEQSV LLLHACAHNP TGVDPRPEQW KEIATVVKKR NLFAFFDMAY QGFASGDGDK DAWAVRHFIE QGINVCLCQS YAKNMGLYGE RVGAFTMVCK DADEAKRVES QLKILIRPMY SNPPLNGARI AAAILNTPDL RKQWLQEVKV MADRIIGMRT QLVSNLKKEG STHNWQHITD QIGMFCFTGL KPEQVERLIK EFSIYMTKDG RISVAGVTSS NVGYLAHAIH QVTK|
|Storage||-20°C; 0.5 mg/ml solution 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 150 mM NaCl and 1 mM DTT.|
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Provided below are standard protocols that you may find useful for product applications.
GOT2, also known as glutamate oxaloacetate transaminase 2, belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Glutamate oxaloacetate transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Obaru K.,et al.J. Biol. Chem. 261:16976-16983(1986).
Tsuzuki T.,et al.J. Mol. Biol. 198:21-31(1987).
Bradbury M.W.,et al.Biochem. J. 345:423-427(2000).
Carninci P.,et al.Science 309:1559-1563(2005).
Lubec G.,et al.Submitted (JAN-2009) to UniProtKB.
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