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IMPAD1, human recombinant protein
Inositol monophosphatase 3, GPAPP, IMP 3, IMP-3, IMPA3
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q9NX62 |
|---|---|
| Concentration | 0.25 |
| Calculated MW | 37.6 kDa (349 aa, 34-359 aa + His Tag) |
| Gene ID | 54928 |
|---|---|
| Gene Symbol | IMPAD1 |
| Other Names | Inositol monophosphatase 3, GPAPP, IMP 3, IMP-3, IMPA3 |
| Gene Source | Human |
| Source | E.coli |
| Assay&Purity | SDS-PAGE; ≥90% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | Specific activity > 3300 pmole/min/ µg |
| Sequence | MGSSHHHHHH SSGLVPRGSH MGSGRFSLFG LGGEPGGGAA GPAAAADGGT VDLREMLAVS VLAAVRGGDE VRRVRESNVL HEKSKGKTRE GAEDKMTSGD VLSNRKMFYL LKTAFPSVQI NTEEHVDAAD QEVILWDHKI PEDILKEVTT PKEVPAESVT VWIDPLDATQ EYTEDLRKYV TTMVCVAVNG KPMLGVIHKP FSEYTAWAMV DGGSNVKARS SYNEKTPRIV VSRSHSGMVK QVALQTFGNQ TTIIPAGGAG YKVLALLDVP DKSQEKADLY IHVTYIKKWD ICAGNAILKA LGGHMTTLSG EEISYTGSDG IEGGLLASIR MNHQALVRKL PDLEKTGHK |
| Target/Specificity | IMPAD1 |
| Format | Liquid |
| Storage | -20°C; 0.25 mg/ml solution in PBS (pH 7.4). |
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Provided below are standard protocols that you may find useful for product applications.
Background
IMPAD1, also known as Inositol monophosphatase 3, is a member of the inositol monophosphatase family. IMPAD1 is localized to the Golgi apparatus and catalyzes the hydrolysis of phosphoadenosine phosphate (PAP) to adenosine monophosphate (AMP). Mutations in this gene are a cause of GRAPP type chondrodysplasia with joint dislocations, and a pseudogene of this gene is located on the long arm of chromosome 1. Recombinant human IMPAD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
References
Parthasarathy L.,et al.Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Frederick J.P.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:11605-11612(2008).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).
Van Damme P.,et al.Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
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