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DUSP18, human recombinant protein
Dual specificity protein phosphatase 18, DUSP20
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q8NEJ0 |
|---|---|
| Concentration | 0.5 |
| Calculated MW | 40.4 kDa (359 aa, 149-482 aa + His Tag) |
| Gene ID | 150290 |
|---|---|
| Gene Symbol | DUSP18 |
| Other Names | Dual specificity protein phosphatase 18, DUSP20 |
| Gene Source | Human |
| Source | E.coli |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | Specific activity: >1,100 units/mg |
| Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMTAPSC AFPVQFRQPS VSGLSQITKS LYISNGVAAN NKLMLSSNQI TMVINVSVEV VNTLYEDIQY MQVPVADSPN SRLCDFFDPI ADHIHSVEMK QGRTLLHCAA GVSRSAALCL AYLMKYHAMS LLDAHTWTKS CRPIIRPNSG FWEQLIHYEF QLFGKNTVHM VSSPVGMIPD IYEKEVRLMI PL |
| Target/Specificity | DUSP18 |
| Format | Liquid |
| Storage | -20°C; 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.1 mM PMSF, 1 mM EDTA and 1 mM DTT |
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Provided below are standard protocols that you may find useful for product applications.
Background
Dual specificity phosphatase 18, also known as DuSP18, is a member of the dual-specificity phosphatase (DSP) family, which catalyzes dephosphorylation of phosphotyrosine and phosphothreonine residues. DuSP18 is inhibited by iodoarectic acid and is activated by manganese ions. Along with having preferential enzymatic activity against phosphorylated tyrosine residues over threonine residues, DuSP18 also dephosphorylates p-nitrophenyl phosphate (pNPP) in vitro. This protein is widely expressed with highest levels in liver, brain, ovary and testis. Recombinant human DuSP18 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
References
Hood K.L.,et al.Biochem. Biophys. Res. Commun. 298:545-551(2002).
Wu Q.,et al.Biochim. Biophys. Acta 1625:296-304(2003).
Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
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