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PGK1, human recombinant protein

Phosphoglycerate kinase 1, MGC117307, MGC142128, MGC8947, MIG10, PGKA

     
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Product info
Primary Accession P00558
Concentration 1
Calculated MW 46.8 kDa (437 aa, 1-417 aa + His Tag)
Additional Info
Gene ID 5230
Gene Symbol PGK1
Other Names Phosphoglycerate kinase 1, MGC117307, MGC142128, MGC8947, MIG10, PGKA
Gene Source Human
Source E.coli
Assay&Purity SDS-PAGE; ≥95%
Assay2&Purity2 N/A;
Recombinant Yes
Results Specific activity is >190 units/mg
Sequence MGSSHHHHHH SSGLVPRGSH MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC LDNGAKSVVL MSHLGRPDGV PMPDKYSLEP VAVELKSLLG KDVLFLKDCV GPEVEKACAN PAAGSVILLE NLRFHVEEEG KGKDASGNKV KAEPAKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPQKAGGFLM KKELNYFAKA LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM AFTFLKVLNN MEIGTSLFDE EGAKIVKDLM SKAEKNGVKI TLPVDFVTAD KFDENAKTGQ ATVASGIPAG WMGLDCGPES SKKYAEAVTR AKQIVWNGPV GVFEWEAFAR GTKALMDEVV KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKVLPG VDALSNI
Target/Specificity PGK1
Format Liquid
Storage -20°C; 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, and 1 mM DTT
Citations (0)

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Background

Phosphoglycerate kinase 1 (PGK1) is an X-linked enzyme that plays a key role in the glycolytic pathway. The gene encoding the erythrocyte enzyme PGK1 and it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. The PGK1 catalyzes the reversible conversion of 1, 3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. Recombinant PGK1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

References

Michelson A.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 80:472-476(1983).
Michelson A.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 82:6965-6969(1985).
Kim J.W.,et al.Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
Shichijo S.,et al.Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 295.00
Cat# PBV11378r
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