|Calculated MW||46.9 kDa (437 aa, 1-417 aa + His Tag)|
|Other Names||Phosphoglycerate kinase 2, dJ417L20.2, PGKB, PGKPSS|
|Results||Specific activity is >11 units/mg|
|Sequence||MGSSHHHHHH SSGLVPRGSH MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM|
|Storage||-20°C; 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1 M NaCl and 1 mM DTT|
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Provided below are standard protocols that you may find useful for product applications.
PGK2 is a testis-specific form of phosphoglycerate kinase. Initially assumed to be a pseudogene, this protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1, 3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis. Recombinant human PGK2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
McCarrey J.R.,et al.Nature 326:501-504(1987).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mungall A.J.,et al.Nature 425:805-811(2003).
Mayya V.,et al.Sci. Signal. 2:RA46-RA46(2009).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).
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