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PGK2, human recombinant protein

Phosphoglycerate kinase 2, dJ417L20.2, PGKB, PGKPSS

     
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Product info
Primary Accession P07205
Concentration 0.5
Calculated MW 46.9 kDa (437 aa, 1-417 aa + His Tag)
Additional Info
Gene ID 5232
Gene Symbol PGK2
Other Names Phosphoglycerate kinase 2, dJ417L20.2, PGKB, PGKPSS
Gene Source Human
Source E.coli
Assay&Purity SDS-PAGE; ≥85%
Assay2&Purity2 N/A;
Recombinant Yes
Results Specific activity is >11 units/mg
Sequence MGSSHHHHHH SSGLVPRGSH MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM
Target/Specificity PGK2
Format Liquid
Storage -20°C; 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1 M NaCl and 1 mM DTT
Citations (0)

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Background

PGK2 is a testis-specific form of phosphoglycerate kinase. Initially assumed to be a pseudogene, this protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1, 3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis. Recombinant human PGK2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

References

McCarrey J.R.,et al.Nature 326:501-504(1987).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mungall A.J.,et al.Nature 425:805-811(2003).
Mayya V.,et al.Sci. Signal. 2:RA46-RA46(2009).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).

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$ 375.00
Cat# PBV11379r-50
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