QDPR, human recombinant protein
Quinoid dihydropteridine reductase, DHPR, FLJ42391, PKU2, SDR33C1
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P09417 |
---|---|
Concentration | 1 |
Calculated MW | 28.2 kDa (267 aa, 1-244 aa + His Tag) |
Gene ID | 5860 |
---|---|
Gene Symbol | QDPR |
Other Names | Quinoid dihydropteridine reductase, DHPR, FLJ42391, PKU2, SDR33C1 |
Gene Source | Human |
Source | E.coli |
Assay&Purity | SDS-PAGE; ≥90% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Results | Specific activity: > 27 units/ml |
Sequence | MGSSHHHHHH SSGLVPRGSH MGSMAAAAAA GEARRVLVYG GRGALGSRCV QAFRARNWWV ASVDVVENEE ASASIIVKMT DSFTEQADQV TAEVGKLLGE EKVDAILCVA GGWAGGNAKS KSLFKNCDLM WKQSIWTSTI SSHLATKHLK EGGLLTLAGA KAALDGTPGM IGYGMAKGAV HQLCQSLAGK NSGMPPGAAA IAVLPVTLDT PMNRKSMPEA DFSSWTPLEF LVETFHDWIT GKNRPSSGSL IQVVTTEGRT ELTPAYF |
Target/Specificity | QDPR |
Format | Liquid |
Storage | -20°C; 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol and 2 mM DTT |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
QDPR is a member of the short-chain dehydrogenases/reductase (SDR) family of enzymes. Functioning as a homodimer, QDPR plays an important role in the recycling of tetrahydrobiopterin (BH4), an essential cofactor for the hydroxylation of the aromatic amino acids (tryptophan, tyrosine and phenylalanine). More specifically, QDPR catalyzes the regeneration of BH4 from quinonoid dihydrobiopterin (qBH2), the product generated from the hydroxylation reactions. Mutations in the gene encoding QDPR can lead to phenylketonuria II. Recombinant human QDPR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
References
Dahl H.-H.M.,et al.Nucleic Acids Res. 15:1921-1932(1987).
Dahl H.-H.M.,et al.Submitted (JUL-1987) to the EMBL/GenBank/DDBJ databases.
Lockyer J.,et al.Proc. Natl. Acad. Sci. U.S.A. 84:3329-3333(1987).
Dianzani I.,et al.Hum. Mutat. 12:267-273(1998).
Hsiao K.-J.,et al.Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.