|Calculated MW||17.2 kDa (152 aa, 1-152 aa)|
|Other Names||Nucleoside diphosphate kinase B, NDPK-B, NDPKB, NM23-H2, NM23B.|
|Results||Specific activity is > 340 units/ml|
|Sequence||MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE|
|Storage||-20°C; 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol and 1 mM DTT|
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Provided below are standard protocols that you may find useful for product applications.
NME2, also known as NM23B, is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways. Recombinant human NME2 protein was expressed in E.coli and purified by using conventional chromatography techniques.
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Postel E.H.,et al.Science 261:478-480(1993).
Valentijn L.J.,et al.Genomics 87:483-489(2006).
Zody M.C.,et al.Nature 440:1045-1049(2006).
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