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NME4, human recombinant protein
Nucleoside diphosphate kinase, mitochondrial, NDK, NDPKD, nm23-H4, NM23D
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | O00746 |
|---|---|
| Concentration | 0.5 |
| Calculated MW | 19.6 kDa (176 aa, 33-187 aa + His Tag) |
| Gene ID | 4833 |
|---|---|
| Gene Symbol | NME4 |
| Other Names | Nucleoside diphosphate kinase, mitochondrial, NDK, NDPKD, nm23-H4, NM23D |
| Gene Source | Human |
| Source | E.coli |
| Assay&Purity | SDS-PAGE; ≥90% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | Specific activity is > 15 units/ml |
| Sequence | MGSSHHHHHH SSGLVPRGSH MPSWTRERTL VAVKPDGVQR RLVGDVIQRF ERRGFTLVGM KMLQAPESVL AEHYQDLRRK PFYPALIRYM SSGPVVAMVW EGYNVVRASR AMIGHTDSAE AAPGTIRGDF SVHISRNVIH ASDSVEGAQR EIQLWFQSSE LVSWADGGQH SSIHPA |
| Target/Specificity | NME4 |
| Format | Liquid |
| Storage | -20°C; 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, and 0.2 M NaCl |
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Provided below are standard protocols that you may find useful for product applications.
Background
NME4, also known as nucleoside diphosphate kinase, mitochondrial, belongs to the NDK family. NME4 are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4. NME4 plays a major role in the synthesis of nucleoside triphosphates other than ATP. Recombinant human NME4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
References
Milon L.,et al.Hum. Genet. 99:550-557(1997).
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Daniels R.J.,et al.Hum. Mol. Genet. 10:339-352(2001).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Martin J.,et al.Nature 432:988-994(2004).
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