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NME4, human recombinant protein

Nucleoside diphosphate kinase, mitochondrial, NDK, NDPKD, nm23-H4, NM23D

     
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Product info
Primary Accession O00746
Concentration 0.5
Calculated MW 19.6 kDa (176 aa, 33-187 aa + His Tag)
Additional Info
Gene ID 4833
Gene Symbol NME4
Other Names Nucleoside diphosphate kinase, mitochondrial, NDK, NDPKD, nm23-H4, NM23D
Gene Source Human
Source E.coli
Assay&Purity SDS-PAGE; ≥90%
Assay2&Purity2 N/A;
Recombinant Yes
Results Specific activity is > 15 units/ml
Sequence MGSSHHHHHH SSGLVPRGSH MPSWTRERTL VAVKPDGVQR RLVGDVIQRF ERRGFTLVGM KMLQAPESVL AEHYQDLRRK PFYPALIRYM SSGPVVAMVW EGYNVVRASR AMIGHTDSAE AAPGTIRGDF SVHISRNVIH ASDSVEGAQR EIQLWFQSSE LVSWADGGQH SSIHPA
Target/Specificity NME4
Format Liquid
Storage -20°C; 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, and 0.2 M NaCl
Citations (0)

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Background

NME4, also known as nucleoside diphosphate kinase, mitochondrial, belongs to the NDK family. NME4 are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4. NME4 plays a major role in the synthesis of nucleoside triphosphates other than ATP. Recombinant human NME4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

References

Milon L.,et al.Hum. Genet. 99:550-557(1997).
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Daniels R.J.,et al.Hum. Mol. Genet. 10:339-352(2001).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Martin J.,et al.Nature 432:988-994(2004).

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$ 295.00
Cat# PBV11385r
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