BLVRA, human recombinant protein
Biliverdin reductase A, BLVR, BVR, BVRA
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P53004 |
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Concentration | 1 |
Calculated MW | 33.3 kDa (295 aa, 3-296 aa) |
Gene ID | 644 |
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Gene Symbol | BLVRA |
Other Names | Biliverdin reductase A, BLVR, BVR, BVRA |
Gene Source | Human |
Source | E.coli |
Assay&Purity | SDS-PAGE; ≥90% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Results | Specific activity is > 30 pmoles/min/ µg. |
Sequence | MAEPERKFGV VVVGVGRAGS VRMRDLRNPH PSSAFLNLIG FVSRRELGSI DGVQQISLED ALSSQEVEVA YICSESSSHE DYIRQFLNAG KHVLVEYPMT LSLAAAQELW ELAEQKGKVL HEEHVELLME EFAFLKKEVV GKDLLKGSLL FTAGPLEEER FGFPAFSGIS RLTWLVSLFG ELSLVSATLE ERKEDQYMKM TVCLETEKKS PLSWIEEKGP GLKRNRYLSF HFKSGSLENV PNVGVNKNIF LKDQNIFVQK LLGQFSEKEL AAEKKRILHC LGLAEEIQKY CCSRK |
Target/Specificity | BLVRA |
Format | Liquid |
Storage | -20°C; 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol |
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Provided below are standard protocols that you may find useful for product applications.
Background
BLVRA, also known as biliverdin reductase A, belongs to the gfo/idh/mocA family. This protein is an enzyme that converts biliverdin to bilirubin, converting a double-bond between the second and third pyrrole ring into a single-bond. (Bilirubin + NAD (P) + = biliverdin + NAD (P) H) Recombinant BLVRA protein was expressed in E.coli and purified by using conventional chromatography techniques.
References
Komuro A.,et al.Biochim. Biophys. Acta 1309:89-99(1996).
Maines M.D.,et al.Eur. J. Biochem. 235:372-381(1996).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 424:157-164(2003).
Maines M.D.,et al.Arch. Biochem. Biophys. 300:320-326(1993).
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