DLD, human recombinant protein
Dihydrolipoyl dehydrogenase, mitochondrial, DLDH, E3, GCSL, LAD, PHE3
|Calculated MW||54.4 kDa (511 aa, 36-509 aa + His Tag)|
|Other Names||Dihydrolipoyl dehydrogenase, mitochondrial, DLDH, E3, GCSL, LAD, PHE3|
|Results||Specific activity: > 50 units/ml|
|Sequence||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMADQ PIDADVTVIG SGPGGYVAAI KAAQLGFKTV CIEKNETLGG TCLNVGCIPS KALLNNSHYY HMAHGKDFAS RGIEMSEVRL NLDKMMEQKS TAVKALTGGI AHLFKQNKVV HVNGYGKITG KNQVTATKAD GGTQVIDTKN ILIATGSEVT PFPGITIDED TIVSSTGALS LKKVPEKMVV IGAGVIGVEL GSVWQRLGAD VTAVEFLGHV GGVGIDMEIS KNFQRILQKQ GFKFKLNTKV TGATKKSDGK IDVSIEAASG GKAEVITCDV LLVCIGRRPF TKNLGLEELG IELDPRGRIP VNTRFQTKIP NIYAIGDVVA GPMLAHKAED EGIICVEGMA GGAVHIDYNC VPSVIYTHPE VAWVGKSEEQ LKEEGIEYKV GKFPFAANSR AKTNADTDGM VKILGQKSTD RVLGAHILGP GAGEMVNEAA LALEYGASCE DIARVCHAHP TLSEAFREAN LAASFGKSIN F|
|Storage||-20°C; 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1 mM DTT and 0.1 M NaCl|
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Provided below are standard protocols that you may find useful for product applications.
DLD (Dihydrolipoamide dehydrogenase), also known as GCSL (glycine cleavage system L protein), is a component of the glycine cleavage system as well as of the alpha ketoacid dehydrogenase complexes. DLD is a flavin-dependent oxidoreductase and functions as a component of the alpha-keto acid dehydrogenase, the pyruvate dehydrogenase, the alpha-ketoglutarate dehydrogenase, the branched-chain alpha-keto acid dehydrogenase and as the L protein in the mitochondrial glycine cleavage system. Mutations in DLD protein can result in MSuD (maple syrup urine disease) and congenital infantile lactic acidosis. Recombinant human DLD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Otulakowski G.,et al.J. Biol. Chem. 262:17313-17318(1987).
Pons G.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:1422-1426(1988).
Feigenbaum A.S.,et al.Genomics 17:376-381(1993).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
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