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HAGH, human recombinant protein
Hydroxyacylglutathione hydrolase, GLO, GLX2, Glyoxalase II, HAGH1
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q16775 |
|---|---|
| Concentration | 0.5 |
| Calculated MW | 31.4 kDa (284 aa, 1-260 aa + His Tag) |
| Gene ID | 3029 |
|---|---|
| Gene Symbol | HAGH |
| Other Names | Hydroxyacylglutathione hydrolase, GLO, GLX2, Glyoxalase II, HAGH1 |
| Gene Source | Human |
| Source | E.coli |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | Specific activity is > 1.8 unit/ml |
| Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMKVEVL PALTDNYMYL VIDDETKEAA IVDPVQPQKV VDAARKHGVK LTTVLTTHHH WDHAGGNEKL VKLESGLKVY GGDDRIGALT HKITHLSTLQ VGSLNVKCLA TPCHTSGHIC YFVSKPGGSE PPAVFTGDTL FVAGCGKFYE GTADEMCKAL LEVLGRLPPD TRVYCGHEYT INNLKFARHV EPGNAAIREK LAWAKEKYSI GEPTVPSTLA EEFTYNPFMR VREKTVQQHA GETDPVTTMR AVRREKDQFK MPRD |
| Target/Specificity | HAGH |
| Format | Liquid |
| Storage | -20°C; 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol |
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Provided below are standard protocols that you may find useful for product applications.
Background
HAGH is a member of the glyoxalase family and a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate. This protein is a detoxifying enzyme of glycolysis byproduct methylglyoxal and a target of p63 and p73 and serves as a pro-survival factor of the p53 family. It exists only as a monomer and binds two zinc ions per subunit. Recombinant human HAGH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
References
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Daniels R.J.,et al.Hum. Mol. Genet. 10:339-352(2001).
Martin J.,et al.Nature 432:988-994(2004).
Ridderstroem M.,et al.J. Biol. Chem. 271:319-323(1996).
Cordell P.A.,et al.J. Biol. Chem. 279:28653-28661(2004).
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