|Calculated MW||This protein rhCEACAM6 is fused with 6×his tag at C-terminus, has a calculated MW of 32 kDa expressed. The predicted N-terminus is Lys35. Protein migrates as 35-65 kDa in reduced SDS-PAGE resulting from glycosylation.|
|Other Names||CEACAM6, CD66c, CEAL, NCA|
|Source||HEK 293 cells|
|Results||Measured by the ability of the immobilized protein to support the adhesion of calcium ionophore treated human neutrophils. When 2 x 105 cells/well are added to CEACAM6 coated plates (10 µg/mL, 100 µL/well), 45-70% of the cells will adhere after 20 minutes at 37ºC.|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.|
|Storage||-20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.|
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Carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen) (CEACAM6) also known as CD66c (Cluster of Differentiation 66c), CEAL, NCA, and is one of seven human CEACAM family members within the immunoglobulin superfamily. In humans, CEACAMs include type I transmembrane proteins (CEACAM1, CEACAM3, and CEACAM4) and GPI-linked molecules (CEACAM5 through CEACAM8). There is no human CEACAM2. CEACAM 6 contains one N-terminal V-type Ig-like domain (N domain), followed by two C2-type Ig-like domains. It shows considerable glycosylation, including (sialyl) LewisX, which mediates binding to E-selectin, galectins and some bacterial fimbrae. CEACAM-6 is expressed by granulocytes and their progenitors. It is also expressed by epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas, as well as hyperplastic polyps. Resistance to adhesion-related apoptosis in tumor cells is conferred in the condition of CEACAM6 overexpression.
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Kalnine N.,et al.Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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