EZCut TEV Protease, recombinant protein
Nuclear inclusion protein A, NIa protein
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Concentration | 1 |
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Calculated MW | 28.6 kDa (2038–2279 aa + C-terminal poly-his tag). |
Other Names | Nuclear inclusion protein A, NIa protein |
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Source | E. coli |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Results | ≥10,000 units/mg |
Sequence | 2038–2279 aa |
Target/Specificity | TEV Protease |
Format | Liquid |
Storage | -80°C; 1 mg/ml solution in 0.1 M Tris-HCl, 0.5 M NaCl, 20% glycerol, 5 mM DTT and 0.5 mM EDTA, pH 8.0 |
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Provided below are standard protocols that you may find useful for product applications.
Background
BioVision’s EZCut™ TEV Protease is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. The optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser/Glycine (ENLYFQS/G). It contains an enhanced form of a catalytic fragment of the NIa protein of Tobacco etch virus (TEV). TEV Protease is a restriction grade protease that has robust activity at 4C with high specificity and great stability. The optimal temperature for cleavage with this enzyme is 34°C. The protease can be used for the removal of affinity tags from fusion proteins. It contains a C-terminal His tag and can be easily removed after cleavage reactions by passing the reaction through a Ni-chelating resin. BioVision’s EZCut™ TEV Protease is an improved version of TEV protease that is highly site-specific, highly active, and significantly more stable than native TEV protease, resulting in enhanced long-term activity.
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