Aprotinin, Active, Bovine recombinant protein (AOF)
BPTI, Basic protease inhibitor, Pancreatic trypsin inhibitor
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P00974 |
---|---|
Calculated MW | 9.7 kDa (36-93 aa + N-terminal Poly-his tag) |
Gene Symbol | BPI |
---|---|
Other Names | BPTI, Basic protease inhibitor, Pancreatic trypsin inhibitor |
Gene Source | Bovine |
Source | E. coli |
Assay&Purity | SDS-PAGE ; ≥90% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Target/Specificity | Aprotinin |
Application Notes | Centrifuge the vial prior to opening at low speed. Reconstitute in water to a concentration of 1 mg/ml. The solution can then be diluted into PBS or other aqueous buffers and store at 4°C for 1 week or –20°C for future use. For long-term storage, it is recommended to add a carrier protein (e.g., 0.1% BSA). Avoid repeated freezing and thawing cycles. |
Format | Lyophilized |
Storage | -20°C; Lyophilized from 5 mg/ml solution in PBS |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Aprotinin inhibits the activity of several proteolytic enzymes such as chymotrypsin, kallikrein, plasmin and trypsin. It is present in blood and in most tissues, with a high concentration in lung, inhibits pro-inflammatory cytokine release and maintains glycoprotein homeostasis. In platelets, Aprotinin reduces glycoprotein loss (e.g., GpIb, GpIIb/IIIa), while in granulocytes it prevents the expression of pro-inflammatory adhesive glycoproteins. Aprotinin is a natural proteinase inhibitor polypeptide consisting of fifty-eight amino acids arranged in a single polypeptide chain, cross-linked by three disulfide bridges. BioVision’s Recombinant Bovine Aprotinin is an animal-origin free protein purified by proprietary chromatographic techniques.
References
Creighton T.E.,et al.J. Mol. Biol. 194:11-22(1987).
Creighton T.E.,et al.Cold Spring Harb. Symp. Quant. Biol. 52:511-519(1987).
Kingston I.B.,et al.Biochem. J. 233:443-450(1986).
Anderson S.,et al.Proc. Natl. Acad. Sci. U.S.A. 80:6838-6842(1983).
Kassell B.,et al.Biochem. Biophys. Res. Commun. 20:463-468(1965).
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.