MMP-9, Active, human recombinant protein
Matrix Metalloproteinase-9, Gelatinase B, 92 kDa Type IV Collagenase, MMP9, CLG4B, GELB, MANDP2
|Calculated MW||39 kDa (aa 107-457 + NT His Tag)|
|Other Names||Matrix Metalloproteinase-9, Gelatinase B, 92 kDa Type IV Collagenase, MMP9, CLG4B, GELB, MANDP2|
|Assay&Purity||SDS-PAGE ; ≥95%|
|Results||> 70 U/ µg|
|Application Notes||Reconstitute with pre-chilled 30% Glycerol solution (in dH2O) to 10 U/ µl and incubate on ice until it completely dissolves. Aliquot and store the reconstituted MMP-9 at -20°C. Stable for 2 months after reconstitution.|
|Storage||-20°C; Lyophilized from a proprietary buffer|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B, is the mostly studied MMP, due to its fundamental role in cancer biology, autoimmune disease, and other conditions. This enzyme degrades various substrates including gelatin, collagen types IV and V, and elastin. MMP-9 is structurally a multi-domain metalloenzyme, composed of a prodomain, a catalytic domain, a gelatin binding domain, a metal-binding domain, and a carboxyl terminal hemopexin like domain. This active human MMP-9 is composed of the catalytic domain, a gelatin binding domain, and a metal binding domain (AA 107-457). The protein was expressed in E.coli and purified and refolded using proprietary techniques.
Wilhelm S.M.,et al.J. Biol. Chem. 264:17213-17221(1989).
Huhtala P.,et al.J. Biol. Chem. 266:16485-16490(1991).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 414:865-871(2001).
Sato H.,et al.Oncogene 8:395-405(1993).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.