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Cytochrome B5A, human recombinant protein
Cytochrome b5, Microsomal cytochrome b5 type A, MCB5, CYB5A, CYB5, Cytochrome B5
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P00167 |
|---|---|
| Concentration | 1 |
| Calculated MW | 15 kDa |
| Gene ID | 1528 |
|---|---|
| Gene Symbol | CYB5A |
| Other Names | Cytochrome b5, Microsomal cytochrome b5 type A, MCB5, CYB5A, CYB5, Cytochrome B5 |
| Gene Source | Human |
| Source | E. coli |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMAEQSD EAVKYYTLEE IQKHNHSKST WLILHHKVYD LTKFLEEHPG GEEVLREQAG GDATENFEDV GHSTDAREMS KTFIIGELHP DDRPKLNKPP ETLITTIDSS SS |
| Target/Specificity | Cytochrome B5A |
| Application Notes | In 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.1 M NaCl and 1 mM DTT |
| Format | Liquid |
| Storage | -20°C; Sterile filtered, 1 mg/ml solution containing 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.1 M NaCl and 1 mM DTT |
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Provided below are standard protocols that you may find useful for product applications.
Background
Cytochrome b5a (CYB5A) is a membrane-bound hemoprotein which functions as an electron carrier for several membrane bound oxygenases like cytochrome P450 enzymes. Defects in the CYB5A gene (METHB-CYB5A) cause methemoglobinemia also known as CYB5A-related methemoglobinemia (a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of blood, cyanosis and hypoxia). BioVision’s human recombinant CYB5A produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids (1-108 a.a of Uniprot Accession# P00167) and having a molecular mass of 14.9kDa. CYB5A is fused to a 24 amino acid long His-tag at the N-terminus & purified by proprietary chromatographic techniques.
References
Yoo M.,et al.Biochem. Biophys. Res. Commun. 156:576-580(1988).
Giordano S.J.,et al.Biochem. Biophys. Res. Commun. 178:38-44(1991).
Li X.R.,et al.Biochem. Biophys. Res. Commun. 209:894-900(1995).
Melton D.,et al.Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
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