Cytochrome B5A, human recombinant protein
Cytochrome b5, Microsomal cytochrome b5 type A, MCB5, CYB5A, CYB5, Cytochrome B5
|Calculated MW||15 kDa|
|Other Names||Cytochrome b5, Microsomal cytochrome b5 type A, MCB5, CYB5A, CYB5, Cytochrome B5|
|Sequence||MGSSHHHHHH SSGLVPRGSH MGSHMAEQSD EAVKYYTLEE IQKHNHSKST WLILHHKVYD LTKFLEEHPG GEEVLREQAG GDATENFEDV GHSTDAREMS KTFIIGELHP DDRPKLNKPP ETLITTIDSS SS|
|Application Notes||In 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.1 M NaCl and 1 mM DTT|
|Storage||-20°C; Sterile filtered, 1 mg/ml solution containing 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.1 M NaCl and 1 mM DTT|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Cytochrome b5a (CYB5A) is a membrane-bound hemoprotein which functions as an electron carrier for several membrane bound oxygenases like cytochrome P450 enzymes. Defects in the CYB5A gene (METHB-CYB5A) cause methemoglobinemia also known as CYB5A-related methemoglobinemia (a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of blood, cyanosis and hypoxia). BioVision’s human recombinant CYB5A produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids (1-108 a.a of Uniprot Accession# P00167) and having a molecular mass of 14.9kDa. CYB5A is fused to a 24 amino acid long His-tag at the N-terminus & purified by proprietary chromatographic techniques.
Yoo M.,et al.Biochem. Biophys. Res. Commun. 156:576-580(1988).
Giordano S.J.,et al.Biochem. Biophys. Res. Commun. 178:38-44(1991).
Li X.R.,et al.Biochem. Biophys. Res. Commun. 209:894-900(1995).
Melton D.,et al.Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.