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Cytochrome B5A, human recombinant protein

Cytochrome b5, Microsomal cytochrome b5 type A, MCB5, CYB5A, CYB5, Cytochrome B5

     
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Product info
Primary Accession P00167
Concentration 1
Calculated MW 15 kDa
Additional Info
Gene ID 1528
Gene Symbol CYB5A
Other Names Cytochrome b5, Microsomal cytochrome b5 type A, MCB5, CYB5A, CYB5, Cytochrome B5
Gene Source Human
Source E. coli
Assay&Purity SDS-PAGE; ≥95%
Assay2&Purity2 N/A;
Recombinant Yes
Sequence MGSSHHHHHH SSGLVPRGSH MGSHMAEQSD EAVKYYTLEE IQKHNHSKST WLILHHKVYD LTKFLEEHPG GEEVLREQAG GDATENFEDV GHSTDAREMS KTFIIGELHP DDRPKLNKPP ETLITTIDSS SS
Target/Specificity Cytochrome B5A
Application Notes In 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.1 M NaCl and 1 mM DTT
Format Liquid
Storage -20°C; Sterile filtered, 1 mg/ml solution containing 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.1 M NaCl and 1 mM DTT
Citations (0)

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Background

Cytochrome b5a (CYB5A) is a membrane-bound hemoprotein which functions as an electron carrier for several membrane bound oxygenases like cytochrome P450 enzymes. Defects in the CYB5A gene (METHB-CYB5A) cause methemoglobinemia also known as CYB5A-related methemoglobinemia (a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of blood, cyanosis and hypoxia). BioVision’s human recombinant CYB5A produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids (1-108 a.a of Uniprot Accession# P00167) and having a molecular mass of 14.9kDa. CYB5A is fused to a 24 amino acid long His-tag at the N-terminus & purified by proprietary chromatographic techniques.

References

Yoo M.,et al.Biochem. Biophys. Res. Commun. 156:576-580(1988).
Giordano S.J.,et al.Biochem. Biophys. Res. Commun. 178:38-44(1991).
Li X.R.,et al.Biochem. Biophys. Res. Commun. 209:894-900(1995).
Melton D.,et al.Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

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$ 4,850.00
Cat# PBV11416r-5
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