|Concentration||5 U/ µl|
|Other Names||T4 DNA Ligase|
|Storage||-80°C; ENZYME STORAGE BUFFER: 20 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.1 mM EDTA, 5 mM DTT, 50% glycerol (v/v).|
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Provided below are standard protocols that you may find useful for product applications.
T4 DNA ligase catalyzes the formation of phosphodiester bonds between 5’-phosphate and 3’-hydroxy ends of double-stranded DNA. Single stranded nicks in double-stranded DNA are also closed by T4 DNA ligase. T4 DNA ligase needs ATP as co-factor.
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