Turbo3C (HRV3C) Protease, recombinant protein
HRV3C Protease
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Calculated MW | 47.0 kDa |
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Other Names | Protease |
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Source | E. coli |
Assay&Purity | SDS-PAGE; |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Format | Liquid |
Storage | -20°C; 2 mg/ml in 25 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM TCEP, and 50% glycerol |
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Provided below are standard protocols that you may find useful for product applications.
Background
BioVision’s Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro, commonly referred to as the PreScission Site. It cleaves between Gln and Gly (independent of Pro). The recombinant form of the HRV3C protease is a restriction grade protease that has robust activity at 4°C with high specific activity and great stability. It does not require any special buffer for its activity and can be used in a buffer most suitable for the target protein. This HRV3C Protease is a 47 kDa protein with both GST and His tags so it can be easily removed by either Ni-chelating or Glutathione (GSH) resin along with the cleaved tag.
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