|Calculated MW||28.9 kDa|
|Other Names||Protease K, Endopeptidase K, Tritirachium alkaline proteinase|
|Application Notes||In 50 mM Tris-HCl, 2 mM Calcium acetate, pH 8.0.|
|Storage||-20°C; Lyophilized with no additives|
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Provided below are standard protocols that you may find useful for product applications.
A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).
Gunkel F.A.,et al.Eur. J. Biochem. 179:185-194(1989).
Jany K.-D.,et al.Biol. Chem. Hoppe-Seyler 367:87-87(1986).
Jany K.-D.,et al.FEBS Lett. 199:139-144(1986).
Jany K.-D.,et al.Biol. Chem. Hoppe-Seyler 366:485-492(1985).
Paehler A.,et al.EMBO J. 3:1311-1314(1984).
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