Human CellExp™ Akt1 Protein (His Tagged & Strep Tagged), human recombinant
RAC-alpha serine/threonine-protein kinase, AKT1, PKB, RAC, RAC-PK-alpha, PKB alpha, Proto-oncogene c
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P31749 |
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Calculated MW | 60 kDa |
Gene ID | 207 |
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Other Names | RAC-alpha serine/threonine-protein kinase, AKT1, PKB, RAC, RAC-PK-alpha, PKB alpha, Proto-oncogene cAkt |
Gene Source | Human |
Source | HEK293 cells |
Assay&Purity | SDS-PAGE;>92% |
Recombinant | Yes |
Sequence | Met 1 - Ala 480 |
Target/Specificity | Akt1 |
Application Notes | Reconstitute in sterile deionized water to a stock solution of 200 µg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% (W/V) HSA or BSA) is recommended for further dilution and long term storage. |
Format | Dry powder |
Storage | +4°C;Lyophilized powder |
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Provided below are standard protocols that you may find useful for product applications.
Background
AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity.
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