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Human CellExpTM Influenza A [A/Hong Kong/483/97(H5N1)] Hemagglutinin (HA), Recombinant
N/A
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q9QSJ8 |
|---|---|
| Calculated MW | This protein is fused with polyhistidine tag at the C-terminus, has a predicted MW of 53.8 kDa. The protein migrates to 80-110 kDa due to glycosylation. |
| Other Names | N/A |
|---|---|
| Gene Source | Influenza A virus |
| Source | HEK 293 cells |
| Assay&Purity | SDS-PAGE;≥95% |
| Recombinant | Yes |
| Sequence | Asp 17 - Gln 531 |
| Target/Specificity | Hemagglutinin (HA) |
| Application Notes | Reconstitute in sterile deionized water to a stock solution of 100 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% (W/V) HSA or BSA) is recommended for further dilution and long term storage. |
| Format | Dry powder |
| Storage | -20°C;Lyophilized powder |
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Provided below are standard protocols that you may find useful for product applications.
Background
Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Hemagglutinin also plays a major role in the determination of host range restriction and virulence. As a class I viral fusion protein, hemagglutinin is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane.
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