|Calculated MW||55.0 kDa|
|Other Names||Exo I|
|Source||E. coli strain containing an overproducing clone of E. coli Exonuclease I.|
|Storage||-20°C;Exonuclease I in 20 mM Tris-HCI (pH 7.5), 5 mM 2-mercaptoethanol, 0.5 mM EDTA, 50% glycerol.|
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Provided below are standard protocols that you may find useful for product applications.
Exonuclease I hydrolyzes single-stranded DNA in the 3'→5' direction, releasing 5'-mononucleotides and leaving the terminal 5'-dinucleotide intact. Hydrolysis is processive and cannot proceed if the 3' terminus is phosphorylated.
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