|Precautions||N-Formyl-Met-Leu-Phe Protein is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Chemotactic factors from both Gram-positive and Gram-negative bacteria are short peptides with N-formyl methionine at the N-terminus (extensively reviewed in reference 1). These peptides are released from bacteria during infection and activate formyl peptide receptor (FPR), a member of G-protein coupled receptors (GPCRs). In human, the FPR family consists mainly of three receptors, FPR1, FPR2/ALX (formerly FPRL1), and FPR3 (formerly FPRL2) which all couple to the Gi subtype of G-proteins and ultimately lead to the activation of phospholipase C and intracellular Ca2+increase1,2.
N-Formyl-Met-Leu-Phe is a selective and potent agonist of the Formylpeptide receptor (FPR1)3.
In human polymorphonuclear leukocytes N-formyl-met-leu-phe activates p38 by a process involving phosphatidylinositol 3-kinase, protein kinase C, and calcium4. N-Formyl-Met-Leu-Phe increased in a dose-dependent manner (0.1 nM – 1 µM) the adherence of neutrophils to vascular endothelial cells which is the initial event in the migration of neutrophils through blood vessel walls to tissue sites of inflammation5.
1 . Ye, R.D. et al.(2009)Pharmacol. Rev.61, 119.
2 . Le, Y. et al.(2002)Trends Immunol. 23, 541.
3 . Showell, H.J. et al. (1976)J. Exp. Med 143,1154.
4 . Krump, E. et al.(1997)J. Biol. Chem.272, 937.
5 . Tonnesen, M.G. et al. (1984)J. Clin. Invest. 74,1581.
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