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Provided below are standard protocols that you may find useful for product applications.
CFSE, also known as 5-(and -6)-Carboxyfluorescein diacetate succinimidyl ester, is a non-fluorescent molecule that easily diffuses across cell membranes. Inside the cell, acetate groups are cleaved by intracellular esterases yielding a fluorescent molecule whose succinimidyl ester group covalently interacts with primary amines of intracellular proteins. CFSE is compatible with standard intracellular staining protocols using aldehyde fixation and saponin-based permeabilization. CFSE is widely used to track cell division and to monitor cell migration in vivo. CFSE can be detected using standard fluorescein filter sets by fluorescence microscopy or flow cytometry.
Lyons AB, Parish CR. 1994. J Immunol Methods. 171(1):131-137. Lyons AB. 2000. J Immunol Methods. 243(1-2):147-154. Miller MJ, Wei SH, Parker I, Cahalan MD. 2002. Science. 296(5574):1869-1873. Parish CR, Glidden MH, Quah BJ, Warren HS. 2009. Curr Protoc Immunol. Chapter 4:Unit4.9.
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