|Application ||FC, FA|
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Provided below are standard protocols that you may find useful for product applications.
Cell Stimulation Cocktail (500X) is a ready to use cocktail that can be used for polyclonal cell activation. It is composed of the phorbol ester, PMA (Phorbol 12-Myristate 13-Acetate), a calcium ionophore (Ionomycin), and the protein transport inhibitors Brefeldin A and Monensin. This Cell Stimulation Cocktail (500X) can be used to elicit cytokine production and retention in a variety of cell types allowing for detection of intracellular protein by flow cytometry.
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