|
Catalog #
|
Applications:
|
Reactivity:
|
Clone Name:
|
|
AM1130a
|
WB, FC, IHC, E
|
H More
|
32AT1672
|
|
Concentration:
|
Size:
|
Accession:
|
Isotype:
|
|
0.25 mg/ml
|
0.1 mg
|
NP_006008, O43490
|
Mouse IgG1
|
|
Target/Specificity:
|
|
This monoclonal antibody is generated from mice immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of AC133.
|
|
Other Names:
|
|
Prominin-like protein 1, Antigen AC133, CD133 antigen, PROM1, PROML1, CD133
|
|
Application Data:
|
|
Calculated MW:
97201 Da
|
|
|
|
|
Western blot analysis of cell lysates from Y79 cell lines using AC133 antibody am1130a. Immunofluorescence analysis of Bone Marrow Mononuclear Cells stained with monoclonal CD133. Image courtesy of Rick Cohen from the Coriell Institute for Medical Research (NJ, USA). |
Localization of PROM1 protein in testicular tissues (A) Positive staining of PROM1 in the stereocilia (arrow) of epididymal duct in an adult. The inset presents a corresponding negative control. (B) Sporadic expression of PROM1 protein was visible in testis with regular spermatogenesis. The arrow points to a spermatogonia with a slightly stained cytoplasm. (C) PROM1 was present in membranous compartment of intratubular tumour cells as marked by the arrow. (D) Solid arrow marks the positive, membranous staining of seminoma cells for PROM1. Note the cytoplasmatic staining in several cells and the positive signal in stromal compartment including leukocytes. (E) Representative staining of PROM1 in testicular tissues from the 22nd week of gestation. The arrows mark the localization of PROM1 in gonocytes to a membrane and cytoplasmatic region. (F) No distinct PROM1 signal could be observed in third trimester of gestation (here, 30th week of gestation is presented) |
|
|
Product Citations:
|
- Yuri Kim, et al. Cancer Res., Dec 2009; 69: 9271 - 9280. . (E-pub)
- Ann. Onc. 2009, et al. Ann. Onc. 2009; 20:viii89-viii106.
- Gashaw, Isabella, et al. Mol. Hum. Reprod. 2007 Oct 01;13(10):721-727.
Data Rewards Program
>
|
|
Application Notes:
|
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunofluorescence | 0.180555556 |
| Immunohistochemistry | |
|
|
Format:
|
|
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
Order Information
List Price (USA): $235.00/0.1mg
Related Products
Data Rewards Program
