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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AM1818a
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WB, IHC, E
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H
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15CT26.4
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Concentration:
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Size:
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Accession:
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Isotype:
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Crude ascites
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0.1 ml
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NP_003757, Q14457
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IgG3λ
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Target/Specificity:
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This antibody was raised using purified His-tagged recombinant full length human Autophagy BECN1.
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Other Names:
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BECN1, ATG6, VPS30, beclin1, ATG6 autophagy related 6 homolog; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein); beclin-1; coiled-coil myosin-like BCL2-interacting protein
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Application Data:
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Calculated MW:
51765 Da
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(LEFT)Western blot analysis of anti-BECN1 Mab (AM1818a) in HepG2, 293, Jurkat and Hela cell line lysates (35ug/lane). BECN1 (arrow) was detected using the Mab ascites (1:2000 dilution).
(RIGHT)Western blot analysis of anti-BECN1 Mab (CA080116A) in recombinant BECN1 protein. BECN1(arrow) was detected using the ascites Mab (1:2000 dilution). |
Breast CA section stained with Autophagy Beclin 1 Antibody Cat. # AM1818a at a 1:50 dilution. Data courtesy of Dr. Anita Thyagarajan, Cancer Research Laboratory, Methodist Research Institute, Indianapolis, Indiana. |
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Product Citations:
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Data Rewards Program
>
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:500-1000 |
| Immunohistochemistry | 1:50-1:200 |
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Format:
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Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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