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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AM7623b
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WB, IHC, E
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H
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48CT12.6.4
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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P29323
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IgG1κ
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Target/Specificity:
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Purified His-tagged EPHB2 protein(Fragment) was used to produced this monoclonal antibody.
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Other Names:
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Ephrin type-B receptor 2; Tyrosine-protein kinase receptor EPH-3; DRT; Receptor protein-tyrosine kinase HEK5; ERK
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Application Data:
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Calculated MW:
117506 Da
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(LEFT)Western blot analysis of anti-EPHB2 Monoclonal Antibody (Cat.#AM7623b) by EPHB2 recombinant protein (Fragment). EPHB2 (Fragment) protein (arrow) was detected using the purified Mab. (1:2000)
(RIGHT)Western blot analysis of EPHB2 (arrow) using mouse monoclonal EPHB2 antibody (Cat.#AM7623b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHB2 gene (Lane 2) (Origene Technologies) |
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EPHB2 Monoclonal Antibody (Cat.#AM7623b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Product Citations:
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Data Rewards Program
>
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:4000 |
| Western blotting | 1:2000 |
| Immunohistochemistry 1:200 | |
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Format:
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Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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