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HA Tag Antibody

Purified Rabbit Polyclonal Antibody (Pab)


specificationbackgroundprotocolsprintmsds
 
Catalog # Applications: Reactivity: Clone Name:
AP1012a WB, IF, ChIP, E N/A  More RB0411
Concentration: Size: Accession: Isotype:
0.25 mg/ml 0.1 mg Rabbit Ig
Target/Specificity:
KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.
Other Names:
Tag from influenza hemagglutinin protein
Application Data:   Calculated MW:  
     
HA Tag Antibody

Western blot analysis of anti-HA tag Pab (Cat. #AP1012a)in HA-tagged recombinant protein bacterial lysate.

HA Tag Antibody

(Top) GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 fibroblasts were transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag.(Provided by Evgeny A. Zemskov, Irina Mikhailenko:Journal of Cell Science 120, 3188-3199 (2007)) (Bottom) ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3-UTR region of GL1 were used in PCR reactions. ACTIN2 provided a control(Provided by Shucai Wang, Su-Hwan Kwak:Development 134, 3873-3882 (2007))

Product Citations:
  1. Jianjun Guo, et al. Plant Cell Physiol. 2009; 50:1681-1694. (Applications: WB)(E-pub)
  2. Marco Scarselli, et al. J. Biol. Chem., Feb 2009; 284: 3577 - 3585. (Applications: IF)
  3. Yidan Lin, et al. Mol. Cancer Ther., Nov 2008; 7: 3556 - 3565. (Applications: WB)
  4. Wang, Shucai, et al. Development 2007 Nov 01;134(21):3873-3882. (Applications: ChIP)
  5. Zemskov, Evgeny A., et al. J. Cell Sci. 2007 Sep 15;120(18):3188-3199. (Applications: IF, WB)
  6. Liu, Xiangguo, et al. Cancer Res. 2006 Dec 01;66(23):11115-11119. (Applications: WB)

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Application Notes:
ELISA1:5,000
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.

 

 

orderOrder Information

in stockList Price (USA): $180.00/0.1mg

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Abgent is the original manufacturer of this product.
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  1. BP1012a:  Blocking Peptide
  2. KT1001a:  Biotin Labeling Kit
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