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Background
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Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion (1). Abgents DYKDDDDK antibody binds to the same epitope recognized by Sigma's Anti-FLAG® antibodies.
(FLAG® is a registered trademark of Sigma-Aldrich Co., which is not affiliated with Abgent).
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Background
References
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- Chubet RG, Brizzard BL. Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells. Biotechniques 1996;20(1):136-141
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