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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP1409a
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WB, IF, IHC, E
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H More
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RB13373
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_000337, P48436
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SOX9.
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Other Names:
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Transcription factor SOX-9
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Application Data:
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Calculated MW:
56137 Da
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(LEFT)Western blot analysis of anti-SOX9 Antibody (N-term) (Cat.#AP1409a) in K562 cell line lysates (35ug/lane). SOX9 (arrow) was detected using the purified Pab (1:60 dilution).
(RIGHT)Western blot analysis of SOX9 (arrow) using rabbit polyclonal SOX9 Antibody (N-term) (Cat.#AP1409a).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX9 gene (Lane 2) (Origene Technologies). |
(LEFT)Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with SOX9 antibody (N-term) (Cat.#AP1409a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
(RIGHT)Immunofluorescence analysis of anti-SOX9 Antibody (N-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI. |
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Product Citations:
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Data Rewards Program
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunofluorescence | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. Then the antibody is purified by peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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