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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP1801a
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WB, IF, IHC, E
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H, M, R, B*, Z*
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RB7609
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_115903, Q9H492
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the N-term of human LC3 (APG8a).
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Other Names:
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Microtubule-associated proteins 1A/1B light chain 3A [Precursor]; Synonyms MAP1A/MAP1B LC3 A; MAP1A/1B light chain 3 A; MAP1 light chain 3-like protein 1; Microtubule-associated protein 1 light chain 3 alpha; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1LC3A
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Application Data:
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Calculated MW:
14272 Da
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TOP: Western blot analysis of anti-LC3 (APG8a) Pab (Cat. #AP1801a) in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).
MIDDLE: Immunofluorescent (IF) staining of mouse brain cells using anti-LC3 (APG8a) Pab (Cat. #AP1801a).
BOTTOM:
LC3 and the CLN3-encoded protein, battenin, co-immunostaining. Wild-type
and mutant cerebellar cells were costained with #AP1801a, anti-LC3 (APG8a) Abgent antibody (Red)
and a monoclonal anti-battenin antibody(Green) and images were
captured by confocal microscopy. Note the numerous larger vesicular structures, typical of
mature AVs, stained with both the LC3 and battenin antibodies in wild-type cells (arrows),
which are rare in mutant cells. Blue=DAPI.
Protocol and data courtesy of Dr. Susan Cotman, Center for Human Genetic Research, Massachusetts General Hospital. |
Formalin-fixed and paraffin-embedded human brain tissue reacted with Autophagy LC3 Antibody (APG8a) (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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