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Autophagy APG4B Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)


specificationbackgroundprotocolsprintmsds
 
Catalog # Applications: Reactivity: Clone Name:
AP1809a WB, IHC, E H, M RB7496
Concentration: Size: Accession: Isotype:
0.25 mg/ml 0.1 mg NP_037457, Q9Y4P1 Rabbit Ig
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human APG4B.
Other Names:
Cysteine protease APG4B; Autophagy 4 homolog B; hAPG4B; Autophagin-1; Autophagy-related cysteine endopeptidase 1; AUT-like 1 cysteine endopeptidase; APG4B; AUTL1, KIAA0943 ; ATG4B
Application Data:   Calculated MW:   44309 Da
     

(LEFT) Western blot analysis of anti-hAPG4B-R31 Pab (Cat. #AP1809a) in 293 cell line lysates transiently transfected with the ATG4B gene (2ug/lane). hAPG4B-R31(arrow) was detected using the purified Pab (1:60 dilution). (RIGHT) The anti-APG4B Pab (Cat. #AP1809a) is used in Western blot to detect APG4B in CEM tissue lysate

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Citations:
  1. Garneau, Hugo, et al. Am J Physiol Gastrointest Liver Physiol 2007 Oct 01;293(4):G758-772.

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Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:100~500
Immunohistochemistry1:50~100
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.

 

 

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in stockList Price (USA): $235.00/0.1mg

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