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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP1809e
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WB, IHC, E
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H, (M), (Z) More
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RB12595
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_037457, Q9Y4P1
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human APG4B.
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Other Names:
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APG4B, AUTL1, MGC1353, APG4 autophagy 4 homolog B; AUT-like 1 cysteine endopeptidase; autophagin-1; autophagy-related cysteine endopeptidase 1; autophagy-related protein 4 homolog B; cysteine protease ATG4B; hAPG4B
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Application Data:
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Calculated MW:
44309 Da
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(Left)Western blot analysis of anti-Autophagy APG4B Antibody (E135) (Cat.#AP1809e) in HL60 cell line lysates (35ug/lane). APG4B(arrow) was detected using the purified Pab (1:60 dilution).
(Right)Western blot analysis of APG4B (arrow) using rabbit polyclonal APG4B Antibody (E135) (Cat.#AP1809e).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APG4B gene (Lane 2) (Origene Technologies). |
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy APG4B antibody (E135)(Cat.#AP1809e), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Product Citations:
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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