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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP1811c
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WB, IHC, E
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H, M*, P*, Pr* More
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RB7567
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_116274, Q86TL0
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the C-term of human APG4D.
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Other Names:
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APG4-D, APG4D, AUTL4, APG4 autophagy 4 homolog D; AUT-like 4 cysteine endopeptidase; AUT-like 4, cysteine endopeptidase; autophagin-4; autophagy-related cysteine endopeptidase 4; autophagy-related protein 4 homolog D; cysteine protease ATG4D; cysteine protease involved in autophagy
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Application Data:
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Calculated MW:
52922 Da
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Western blot analysis of APG4D Pab (Cat. #AP1811c). LEFT: Y79 cell lysate. RIGHT: cos7, HEK293, MEF, and Hela cells, left to right respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Product Citations:
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Data Rewards Program
>
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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