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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP1812a
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WB, IHC, E
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H, M, B*, P*, R*, Z* More
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RB7466
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_004840, Q9H1Y0
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the N-term of human APG5L.
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Other Names:
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APG5, APG5-LIKE, APG5L, ASP, hAPG5, APG5 (autophagy 5, S. cerevisiae)-like; APG5 autophagy 5-like; ATG5 autophagy related 5-like; OTTHUMP00000017824; apoptosis specific protein; apoptosis-specific protein; autophagy protein 5
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Application Data:
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Calculated MW:
32447 Da
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(LEFT) The anti-APG5L Pab (Cat. #AP1812a) is used in Western blot to detect APG5L in mouse brain tissue lysate. (RIGHT) Western blot analysis of anti-APG5L antibody (Cat. #AP1812a) in Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research. |
(TOP)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
(BUTTOM)Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Autophagy APG5L antibody (N-term) (Cat.#AP1812a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Product Citations:
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Data Rewards Program
>
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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