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Autophagy APG7L Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)


specificationbackgroundprotocolsprintmsds
 
Catalog # Applications: Reactivity: Clone Name:
AP1813b WB, IHC, E H, M, C*, R* RB7469
Concentration: Size: Accession: Isotype:
0.25 mg/ml 0.1 mg NP_006386, O95352 Rabbit Ig
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the Center of human APG7L.
Other Names:
Autophagy protein 7-like; APG7-like; Ubiquitin activating enzyme E1-like protein; hAGP7; APG7L; ATG7
Application Data:   Calculated MW:   77960 Da
     

(LEFT) Western blot analysis of anti-hAPG7L-P299 Pab (Cat. #AP1813b) in 293 cell line lysates transiently transfected with the ATG7 gene (2ug/lane). hAPG7L-P299(arrow) was detected using the purified Pab (1:30 dilution). (RIGHT) The anti-APG7L Pab (Cat. #AP1813c) is used in Western blot to detect APG7L in mouse liver tissue lysate. APG7L (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:100~500
Immunohistochemistry1:50~100
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.

 

 

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in stockList Price (USA): $235.00/0.1mg

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