Autophagy APG16L Antibody (L176)

Western blot of APG16L (L92) Pab (Cat. #AP1817b) LEFT: Mouse brain tissue lysate. RIGHT: cos7, HEK293, MEF, and Hela cells, left to right, respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.

UPPER: Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L antibody (L176), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. LOWER: Paraformaldehyde-fixed paraffin-embedded biopsy from patient with confirmed colonic Crohn disease. Slide subjected to a citrate-based antigen retrieval procedure, permeabilized by incubation with 0.1% Triton X-100 in 0.1M PBS) washed three times in PBS and blocked with 0.75% bovine serum albumin in PBS for 20 minutes. Sections subsequently incubated with APG16L primary antibody at a 1:200 dilution in 0.75% BSA for 1 h RT. Data courtesy of J. Hampe, S. Schreiber, P. Rosenstiel et al., Institute for Clinical Molecular Biology and First Department of Medicine, Christian-Albrechts University Kiel, University Hospital Schleswig-Holstein, 24105 Kiel, Germany.