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EIF4B Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)


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Catalog # Applications: Reactivity: Clone Name:
AP1950c WB, IHC, E H RB11720
Concentration: Size: Accession: Isotype:
0.25 mg/ml 0.1 mg NP_001408, Q8WYK5 Rabbit Ig
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human EIF4B Antibody.
Other Names:
Application Data:   Calculated MW:   69151 Da
     

(LEFT)Western blot analysis of anti-EIF4B Antibody (Center) Pab (Cat.#AP1950c) in HepG2 cell line lysates (35ug/lane). EIF4B(arrow) was detected using the purified Pab (1:60 dilution). (RIGHT)Western blot analysis of anti-EIF4B Antibody (Center) Pab (Cat.#AP1950c) in 293 cell line lysates transiently transfected with the EIF4B gene (2ug/lane). EIF4B(arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4B antibody (Center)(Cat.#AP1950c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:50~100
Immunohistochemistry1:50-100
ImmunoprecipitationNot tested
Flow cytometryNot tested
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.

 

 

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in stockList Price (USA): $235.00/0.1mg

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Abgent is the original manufacturer of this product.
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