|
Catalog #
|
Applications:
|
Reactivity:
|
Clone Name:
|
|
AP1981b
|
WB, IHC, E
|
H, (M) More
|
RB11405
|
|
Concentration:
|
Size:
|
Accession:
|
Isotype:
|
|
0.25 mg/ml
|
0.1 mg
|
NP_004086
|
Rabbit Ig
|
|
Target/Specificity:
|
|
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T36 of human EIF4EBP1.
|
|
Other Names:
|
|
4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
|
|
Application Data:
|
|
Calculated MW:
12449 Da
|
|
|
|
|
Western blot analysis of EIF4EBP1 (arrow) using rabbit polyclonal EIF4EBP1 Antibody (T36) (RB11405). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4EBP1 gene (Lane 2) (Origene Technologies). |
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with EIF4EBP1 Antibody (T36) (Cat.#AP1981b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
|
|
Product Citations:
|
|
Data Rewards Program
>
|
|
Application Notes:
|
| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
|
|
Format:
|
|
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|